![]() The threshold phosphatase level increases with increasing basal level of σ B operon transcription (Basal B T). σ B pulse amplitudes show a threshold linear response to increase in phosphatase level. The large step-increase in phosphatase (C) does significantly shift the post-translational response around the initial steady state leading to prominent pulse in σ B (blue curve in C). The small step-increase in phosphatase does not significantly shift the post-translational response around the initial steady state leading to minor, transient increase in σ B (orange curve in B). Note that at the initial phosphatase level the σ B~0 and B T is at the basal level of σ B operon transcription. Black and gray circles mark the steady states of the full system. Black curves show the transcriptional response. Cyan and green curves show the post-translational responses at initial and final phosphatase levels. Representation of the σ B pulse trajectories and decoupled post-translational and transcriptional responses of σ B network for small (B) and large (C) step-increases in phosphatase. ![]() λ W = 4, λ V = 4.5 for both trajectories. Time-course representations of σ B pulse trajectories for small (0.1μM-orange curve) and large (0.4μM-blue curve) step-increases in phosphatase. S5 Fig: Dependence of σ B pulse amplitude on phosphatase concentrations and post-translational parameters. ![]()
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